L-Aspartic acid-[13C4,15N,d3]

L-Aspartic acid-[13C4,15N,d3], a labeled amino acid widely employed in scientific research, serves as a versatile tool for exploring metabolic pathways, unraveling protein structures, and more. Here are the key applications of L-Aspartic acid-[13C4,15N,d3], delivered with a high degree of perplexity and burstiness:

Metabolic Pathway Tracing: Embarking on metabolic studies, researchers utilize L-Aspartic acid-[13C4,15N,d3] as a tracer to meticulously trace the incorporation and transformation of amino acids within organisms. By harnessing this labeled compound, scientists gain intricate insights into metabolic fluxes and pathway kinetics, paving the way for a deeper understanding of disease mechanisms and the tailored development of metabolic therapies.

Protein Structure and Dynamics: At the forefront of scientific exploration, the labeled isotopes within L-Aspartic acid-[13C4,15N,d3] play a pivotal role in nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry for unraveling protein structures. Through the integration of this labeled amino acid into proteins, researchers delve into the 3D conformation and dynamics of protein molecules with surgical precision. This endeavor is paramount for drug discovery and the deciphering of protein functions on a molecular scale.

Stable Isotope Labeling in Cultivation (SILAC): In the realm of SILAC experiments, L-Aspartic acid-[13C4,15N,d3] emerges as a cornerstone for quantifying protein expression and turnover in cell culture systems. Through nuanced labeling strategies, researchers compare the proteomes of cells under diverse conditions, unraveling insights into cellular responses and regulatory mechanisms. This methodology proves indispensable for delving into cancer biology, immunology, and other fields necessitating meticulous proteomic analyses.

Environmental and Ecological Research: Within ecological inquiries, L-Aspartic acid-[13C4,15N,d3] finds application in tracking nutrient cycles and energy flows within ecosystems. By following the path of this labeled compound, researchers monitor the cycling of amino acids through various trophic levels and environmental compartments, shedding light on ecological interactions and the repercussions of environmental shifts on nutrient dynamics.