L-Aspartic acid-[2,3,3-d3,15N]

L-Aspartic acid-[1,4-13C2,15N] a stable isotope-labeled compound is extensively utilized in biochemical and pharmaceutical research. Here are the key applications of L-Aspartic acid-[1,4-13C2,15N]:

Metabolic Flux Analysis: Delving into the intricate pathways of cellular metabolism L-Aspartic acid-[1,4-13C2,15N] serves as a powerful tool for tracing metabolic fluxes within living cells. By integrating this labeled compound into metabolic processes researchers can meticulously monitor the flow and transformation of metabolic intermediates. This data plays a pivotal role in unraveling the complexities of cellular metabolism shedding light on potential targets for therapeutic interventions.

Protein Structure and Function Studies: Through isotope labeling of L-Aspartic acid researchers embark on a journey of precise exploration into protein structures and dynamics using advanced techniques like NMR spectroscopy. By pinpointing the incorporation of the labeled amino acid into specific protein domains scientists glean deep insights into protein folding dynamics stability and interactions. These studies are of paramount importance in the realm of drug design and unravelling the nuances of protein dysfunctions linked to various diseases.

Enzyme Mechanism Elucidation: Embracing the power of L-Aspartic acid-[1,4-13C2,15N] researchers dive into the realm of enzyme mechanisms and catalytic processes. Through the incorporation of this labeled substrate into enzymatic reactions scientists embark on a journey to unveil isotope effects offering intricate details about transition states and structures of reaction intermediates. This knowledge plays a crucial role in designing enzyme inhibitors and optimizing biocatalysts for industrial applications.

Dynamic Isotope Labeling in Cells: Revolutionizing cellular studies L-Aspartic acid-[1,4-13C2,15N] is a cornerstone of dynamic labeling experiments aimed at dissecting cellular processes over time. By meticulously monitoring the integration and turnover of the isotope-labeled amino acid scientists illuminate the dynamics of cellular growth protein synthesis rates and turnover dynamics. Such studies are imperative for grasping the intricacies of cell physiology and comprehending the impacts of diverse treatments on cellular well-being.