L-Azidohomoalanine-[1,2,3,4-13C4,2,4-15N2] Hydrochloride

L-Azidohomoalanine-[1,2,3,4-13C4,2,4-15N2] Hydrochloride, a labeled amino acid widely utilized in biochemical and molecular biology research, offers a plethora of applications showcased in high perplexity and burstiness:

Protein Labeling and Tracking: Applied in live cell settings, L-Azidohomoalanine serves as a pivotal tool for labeling newly synthesized proteins, enabling researchers to meticulously monitor protein synthesis and turnover dynamics. By integrating this non-natural amino acid into proteins, scientists can utilize click chemistry to affix fluorescent tags or other probes, illuminating intricate protein behaviors, interactions, and cellular distribution dynamics in real-time.

Metabolic Labeling: In the domain of metabolic labeling investigations, this compound plays a crucial role in exploring protein synthesis and cellular metabolic processes. By supplying cells with L-Azidohomoalanine, researchers can track the integration of the labeled amino acid into proteins over time, shedding light on protein expression patterns and responses to diverse stimuli like drug treatments or environmental challenges.

Proteomics Research: A cornerstone in proteomics applications, L-Azidohomoalanine stands out for its role in identifying and quantifying newly synthesized proteins. The azide group facilitates the selective enrichment of labeled proteins through bioorthogonal click chemistry reactions, paving the way for mass spectrometry-based analyses that deepen our comprehension of proteome dynamics and the functional implications of proteins.

Biomedical Imaging: In the realm of biomedical imaging innovations, the utility of L-Azidohomoalanine shines through its ability to visualize protein synthesis within tissues and whole organisms. By incorporating this labeled amino acid into proteins, researchers can leverage advanced imaging modalities such as positron emission tomography (PET) or fluorescence microscopy to capture biological processes in vivo. This application holds immense significance in unraveling developmental biology intricacies, disease progression trajectories, and evaluating the impacts of therapeutic interventions.