L-Cysteine-[1-13C,15N]

L-Cysteine-[1-13C,15N] is an isotopically labeled amino acid used in various scientific investigations. Here are some key applications of L-Cysteine-[1-13C,15N]:

Metabolic Flux Analysis: L-Cysteine-[1-13C,15N] is employed in metabolic flux analysis to trace metabolic pathways and quantify fluxes within cells. By incorporating this labeled cysteine into cellular systems, researchers can follow the incorporation of 13C and 15N isotopes using mass spectrometry or NMR. This provides detailed insights into cellular metabolism and helps in understanding metabolic alterations in different conditions.

Protein Structure and Dynamics: L-Cysteine-[1-13C,15N] is used in NMR spectroscopy to study protein structure, folding, and dynamics. The isotopic labels provide distinctive signals that allow scientists to analyze the positioning and movement of cysteine residues within proteins. This information is crucial for elucidating protein function and designing therapeutic interventions targeting specific protein sites.

Environmental and Ecological Studies: This labeled cysteine is used to trace sulfur and nitrogen cycles in environmental and ecological research. By adding L-Cysteine-[1-13C,15N] to soil or aquatic systems, scientists can monitor the assimilation and transformation of these elements in microbes and plants. This helps in understanding nutrient cycling and ecosystem dynamics.

Pharmaceutical Research: L-Cysteine-[1-13C,15N] is utilized in the development and testing of new drugs, especially those targeting sulfhydryl groups. The isotopic labeling allows precise tracking of cysteine involvement in drug interactions and metabolic transformations. This insight is valuable for optimizing drug design and predicting potential metabolic pathways involved in drug metabolism.