L-Glutamic acid-[4-13C]

L-Glutamic acid-[4-13C], a labeled amino acid, is a versatile compound widely utilized in scientific research due to its stable carbon isotope. Here are the key applications of L-Glutamic acid-[4-13C]:

Metabolic Flux Analysis: Embedded in metabolic flux analysis, L-Glutamic acid-[4-13C] serves as a crucial tool for tracing carbon flux within cellular pathways. By integrating this labeled amino acid, researchers can meticulously monitor the utilization and processing of glutamic acid within intricate metabolic networks. This deepens our comprehension of cell metabolism, refines bioprocess optimization, and pinpoints metabolic bottlenecks.

NMR Spectroscopy: Utilized as a probe in nuclear magnetic resonance (NMR) spectroscopy, L-Glutamic acid-[4-13C] plays a pivotal role in investigating protein structure and dynamics. The presence of the stable carbon isotope yields a distinctive signal in NMR spectra, facilitating a detailed exploration of molecular interactions and conformational changes. This application is indispensable in the realms of structural biology and drug design.

Stable Isotope Labeling: As a valuable asset in stable isotope labeling experiments, L-Glutamic acid-[4-13C] is frequently paired with mass spectrometry. Researchers leverage this labeled amino acid to quantify protein turnover rates and examine post-translational modifications. Such studies are paramount for elucidating protein function, unraveling signaling pathways, and dissecting disease mechanisms.

Enzyme Kinetics: In the domain of enzyme kinetics research, L-Glutamic acid-[4-13C] emerges as a potent tool for scrutinizing the catalytic mechanisms of enzymes utilizing glutamic acid as a substrate. The presence of the labeled carbon enables researchers to trace reaction intermediates and determine essential kinetic parameters. This knowledge is pivotal for informed enzyme engineering and the design of enzyme inhibitors, driving progress in enzyme-related fields.