L-Glutamine-[13C5,15N2,d10]

L-Glutamine-[13C5,15N2,d10], an isotopically labeled variant of the amino acid glutamine, serves as a powerful tool in scientific research, enabling exploration into metabolic pathways and protein dynamics. Here are key applications of L-Glutamine-[13C5,15N2,d10] presented with high perplexity and burstiness:

Metabolic Flux Analysis: In the realm of metabolic research, L-Glutamine-[13C5,15N2,d10] emerges as a pivotal tracer for metabolic flux analysis. By introducing this labeled amino acid into cellular systems, scientists can meticulously monitor its integration into diverse metabolic pathways. This intricate technique allows for the precise quantification of carbon and nitrogen atom flow across cellular processes, offering profound insights into the intricacies and regulatory mechanisms of metabolic networks.

Protein Turnover Studies: Delving into the realm of protein dynamics, L-Glutamine-[13C5,15N2,d10] plays a crucial role in examining protein synthesis and degradation rates within cells. Through the measurement of isotopically labeled glutamine incorporation into proteins, researchers uncover the dynamic patterns of protein turnover. Such investigations are essential for unraveling cellular responses under varied physiological conditions and probing the impacts of diseases on protein metabolism with a fine-tooth comb.

Cancer Metabolism Research: In the context of cancer biology, L-Glutamine-[13C5,15N2,d10] serves as a cornerstone in unraveling the intricacies of altered glutamine metabolism within tumor cells. Given the tendency of cancer cells to exhibit heightened glutamine uptake and utilization, this labeled compound becomes indispensable for tracing the paths of glutamine-derived carbon and nitrogen. This groundbreaking research aids in pinpointing potential metabolic vulnerabilities in cancerous cells, paving the way for targeted therapeutic interventions that hold the promise of revolutionizing cancer treatment paradigms.

Stable Isotope-Resolved Metabolomics: At the forefront of metabolomics, L-Glutamine-[13C5,15N2,d10] is a key player in stable isotope-resolved metabolomics, offering a comprehensive lens into cellular metabolism. By seamlessly integrating this labeled glutamine into metabolic inquiries, researchers gain a nuanced understanding of pathway preferences and fluxes. This cutting-edge approach enhances insights into metabolic alterations across diverse physiological and pathological contexts, fueling the advancement of novel diagnostic tools and innovative treatment strategies.