L-Glutamine-[2,3,3,4,4-D5,15N2]

L-Glutamine-[2,3,3,4,4-D5,15N2], a stable isotope-labeled compound widely used in biotechnology and research, finds diverse applications in various fields. Here are the key applications of this isotopically labeled glutamine:

Metabolic Flux Analysis: Immersing into the intricate metabolic pathways of microbial and mammalian cells, researchers rely on L-Glutamine-[2,3,3,4,4-D5,15N2] to meticulously track and quantify fluxes within these systems. By integrating this labeled compound, scientists can meticulously follow the convoluted journey of glutamine through cellular metabolism, shedding light on energy production and metabolic complexities. This knowledge plays a pivotal role in deciphering diseases such as cancer, where metabolic processes frequently deviate from the norm.

Protein Biosynthesis Studies: In the domain of protein synthesis and turnover investigations, L-Glutamine-[2,3,3,4,4-D5,15N2] takes the spotlight. By introducing this specially labeled glutamine into cell cultures, researchers can meticulously monitor how glutamine integrates into newly synthesized proteins using cutting-edge mass spectrometry techniques. This methodology offers profound insights into protein dynamics and the impact of various treatments on protein production and degradation, enriching our understanding of cellular processes.

Cell Cycle and Proliferation Research: Unveiling the mysteries of cell growth and division, particularly in the context of cancer cells heavily reliant on glutamine as a vital nutrient, L-Glutamine-[2,3,3,4,4-D5,15N2] emerges as a crucial tool. By labeling the glutamine pool, researchers embark on a journey to explore how cells harness this amino acid during different phases of the cell cycle, providing critical insights for the development of anti-cancer therapies that target metabolic vulnerabilities.

Immunology Studies: In the realm of immunological exploration, L-Glutamine-[2,3,3,4,4-D5,15N2] acts as a gateway to understanding the pivotal role of glutamine in immune cell function and response dynamics. Acknowledged for its significance in the proliferation and functionality of immune cells like lymphocytes and macrophages, glutamine undergoes intense scrutiny in these studies. By employing the labeled compound, researchers illuminate the intricate landscape of glutamine metabolism within immune cells, enriching our comprehension of immune regulation and activation processes.