Boc-Gln-OH-[alpha-15N]

Boc-Gln-OH-[alpha-15N], a labeled amino acid derivative, plays a pivotal role in scientific research, particularly in investigations concerning protein synthesis and structural biology. Here are key applications, presented with high perplexity and burstiness:

Nuclear Magnetic Resonance (NMR) Spectroscopy: In the realms of NMR spectroscopy, Boc-Gln-OH-[alpha-15N] emerges as a valuable tool for examining the structures and dynamics of proteins. The 15N label acts as a sensitive probe, allowing researchers to delve into the amide nitrogen environments within proteins. Detailed insights into molecular conformation and interactions are thereby gleaned, shedding light on the intricate functionalities of proteins.

Proteomics Research: Within the domain of proteomics, Boc-Gln-OH-[alpha-15N] assumes the role of an isotopic label, facilitating the quantification of proteins and the analysis of post-translational modifications. The incorporation of the 15N label enables precise mass spectrometry-based quantification, enabling the comparative assessment of protein abundance across diverse samples. This methodology proves invaluable for scrutinizing cellular responses and deciphering disease mechanisms at the proteome level.

Protein Synthesis Studies: Delving into the intricacies of protein synthesis, Boc-Gln-OH-[alpha-15N] finds application in studies dedicated to unraveling translation mechanisms and amino acid integration. Through the utilization of labeled glutamine, researchers can track its integration into newly synthesized proteins, unveiling insights into the efficiency and fidelity of the translation process. This exploration sheds light on genetic expression and cellular functionalities, enriching our understanding of genetic processes.

In Vivo Metabolic Tracing: In the realm of metabolic inquiries, Boc-Gln-OH-[alpha-15N] emerges as a valuable tracer for nitrogen metabolism within living organisms. The 15N label facilitates the monitoring of nitrogen flow through metabolic pathways, aiding in the comprehension of amino acid utilization and nitrogen equilibrium. This investigative approach proves essential for unraveling nutrient assimilation and metabolic regulation across diverse biological systems, offering key insights into physiological processes.