L-Histidine-[alpha-15N] hydrochloride monohydrate

L-Histidine-[alpha-15N] hydrochloride monohydrate, a stable isotope-labeled amino acid, finds utility in a range of advanced research realms. Explore its diverse applications:

Metabolic Tracing: Delving into metabolic tracing studies, scientists harness L-Histidine-[alpha-15N] hydrochloride monohydrate to trace the incorporation and transformation of histidine within biochemical pathways. By monitoring the destiny of the labeled nitrogen atom, researchers glean profound insights into histidine metabolism and its intricate role in cellular physiology. This data stands as a cornerstone for unraveling metabolic disorders and crafting precise therapeutic interventions.

Protein Structure Analysis: Embracing the realm of protein structure analysis, the isotope-labeled L-Histidine-[alpha-15N] emerges as a potent tool in nuclear magnetic resonance (NMR) spectroscopy for scrutinizing protein structural nuances and dynamics. Through integrating this labeled amino acid into proteins, scientists glean intricate details about protein folding, interactions, and conformational transitions. This knowledge fuels drug design endeavors and sheds light on the intricate dance of protein functionality.

Enzyme Mechanism Studies: Unraveling enzyme mechanisms, L-Histidine-[alpha-15N] hydrochloride monohydrate serves as a pivotal player in probing how the labeled nitrogen atom engages in catalytic reactions. Leveraging techniques like NMR and mass spectrometry, researchers witness enzymatic processes involving histidine residues firsthand. This application holds the key to identifying critical functional residues and deciphering the enigmatic realm of catalytic phenomena.

Nutritional Research: In the domain of nutritional inquiries, L-Histidine-[alpha-15N] takes center stage in exploring amino acid absorption, utilization, and nitrogen dynamics within living organisms. By administering the labeled amino acid, scientists monitor histidine metabolism and its contributions to overall protein and nitrogen utilization. This avenue of research plays a vital role in optimizing dietary strategies and unraveling the intricate tapestry of nutritional demands in living organisms.