L-Histidine-[d5,15N3] hydrochloride monohydrate

L-Histidine-[d5,15N3] hydrochloride monohydrate is a labeled amino acid used in various research fields, providing insights into biological processes. Here are some key applications of L-Histidine-[d5,15N3] hydrochloride monohydrate:

Stable Isotope Labeling: L-Histidine-[d5,15N3] hydrochloride monohydrate is used in stable isotope labeling experiments for protein structure and dynamics studies. This compound allows researchers to trace histidine residues within proteins through techniques such as nuclear magnetic resonance (NMR) spectroscopy. It plays a critical role in understanding protein folding, interactions, and conformational changes.

Metabolic Flux Analysis: The labeled L-Histidine enables detailed examination of metabolic pathways in organisms. By tracking the incorporation of this compound, scientists can quantify and map histidine's role in various metabolic routes. This information is essential for understanding amino acid metabolism and its implications for cell physiology and pathology.

Pharmaceutical and Nutritional Research: L-Histidine-[d5,15N3] hydrochloride monohydrate can be utilized to explore the biochemical pathways related to histidine in drug and nutrient metabolism. It provides insights into histidine's involvement in enzymatic reactions and its significance in health and disease. This knowledge aids in designing therapeutics and dietary strategies for conditions linked to histidine metabolism.

Protein Engineering: In the field of protein engineering, this labeled compound is used to investigate histidine's role in catalytic sites and binding domains. By incorporating this isotope-labeled histidine into engineered proteins, researchers can gain a detailed understanding of enzyme mechanisms and improve protein design. These findings are integral for developing biocatalysts and therapeutic proteins.