L-Histidine-[ring-2-13C] hydrochloride monohydrate

L-Histidine-[ring-2-13C] hydrochloride monohydrate is a labeled amino acid used in various advanced research applications. Here are some key applications of L-Histidine-[ring-2-13C] hydrochloride monohydrate:

Protein Structure and Dynamics: L-Histidine-[ring-2-13C] hydrochloride monohydrate is employed in NMR (Nuclear Magnetic Resonance) spectroscopy to study protein structures and dynamics. The incorporation of the 13C isotope into histidine residues allows researchers to gain detailed insights into protein folding interactions and conformational changes. This is crucial for understanding the fundamental principles of protein function and stability.

Metabolic Flux Analysis: In metabolic studies, L-Histidine-[ring-2-13C] hydrochloride monohydrate is used to trace metabolic pathways and fluxes. By integrating this labeled histidine into metabolic models, scientists can monitor its incorporation into metabolic products. This aids in elucidating the metabolic fate of histidine and contributes to the better understanding of metabolic networks.

Stable Isotope Labeling for Mass Spectrometry: This compound is utilized in mass spectrometry-based proteomics for stable isotope labeling. By incorporating L-Histidine-[ring-2-13C] into proteins, researchers can quantify protein abundance and turnover rates with high precision. This technique is valuable for studying protein expression changes and post-translational modifications in various biological contexts.

Enzyme Mechanism Studies: L-Histidine-[ring-2-13C] hydrochloride monohydrate is used to investigate enzyme mechanisms that involve histidine residues. The 13C labeling helps in identifying and characterizing active site residues and intermediate states during enzyme catalysis. This information is critical for unraveling enzyme functions and designing inhibitors for therapeutic applications.