L-Histidine-[ring-pi-15N] hydrochloride monohydrate

L-Histidine-[ring-pi-15N] hydrochloride monohydrate, an isotopically labeled amino acid with specific applications in bioscience and medical research, offers a myriad of possibilities. Here are the key applications described with a high degree of perplexity and burstiness:

Metabolic Tracing: Dive into the intricate world of amino acid metabolism and protein synthesis pathways with the aid of L-Histidine-[ring-pi-15N] hydrochloride monohydrate in metabolic tracing studies. By introducing this labeled histidine into cells or organisms, researchers embark on a journey to unveil its metabolic destiny through the lens of mass spectrometry or NMR spectroscopy. This exploration sheds light on metabolic fluxes and the intricate role of histidine in a multitude of physiological processes, unraveling the complexities of cellular function.

Protein Structure and Dynamics: Embark on a voyage of discovery in structural biology using L-Histidine-[ring-pi-15N] hydrochloride monohydrate for NMR-based investigations of protein structure and dynamics. The isotopic labeling amplifies NMR signals, enabling a meticulous examination of histidine residues within protein systems. This deeper analysis provides profound insights into protein folding dynamics, molecular interactions, and conformational changes pivotal for unraveling the mysteries of protein functionality and behavior in diverse cellular processes.

Isotope-Labeling in Drug Development: Delve into the realm of pharmaceutical research with the aid of L-Histidine-[ring-pi-15N] hydrochloride monohydrate, a valuable ally in studying drug-protein interactions and pharmacokinetics. By incorporating the labeled histidine into target proteins, researchers embark on a journey to unveil the intricate dance between drugs and binding sites using NMR and other cutting-edge techniques. This knowledge forms the cornerstone for designing potent, targeted therapeutic agents that hold the promise of revolutionizing drug development and personalized medicine.

Biosynthetic Pathway Elucidation: Explore the enigmatic world of biosynthetic pathways of histidine in microorganisms and plants with the aid of L-Histidine-[ring-pi-15N] hydrochloride monohydrate. By providing the isotopically labeled precursor, scientists trace its incorporation into intermediate and final products of the biosynthesis pathway, uncovering the molecular choreography underlying histidine biosynthesis. This journey unveils the enzymatic steps, regulatory mechanisms, and metabolic crossroads, paving the way for advancements in metabolic engineering and synthetic biology, offering new avenues for bioengineering breakthroughs.