L-Isoleucine-[1-13C]

L-Isoleucine-[1-13C] is a stable isotope-labeled amino acid commonly used in various bioscience applications. Here are some key applications of L-Isoleucine-[1-13C]:

Metabolic Flux Analysis: L-Isoleucine-[1-13C] is widely used to study metabolic fluxes in cellular systems. By tracing the incorporation of the labeled carbon atom, researchers can map metabolic pathways and understand the dynamic changes in metabolism. This information is crucial for optimizing industrial fermentation processes and developing therapeutic strategies for metabolic disorders.

Protein Structure Elucidation: In the field of structural biology, L-Isoleucine-[1-13C] is employed in NMR spectroscopy to study protein structures and dynamics. The isotope label provides unique resonance signals that help in identifying the position and environment of the amino acid within the protein. This enables scientists to gain detailed insights into protein folding, conformational changes, and interactions with other molecules.

Kinetic Studies: L-Isoleucine-[1-13C] is used to investigate enzyme kinetics and reaction mechanisms. By incorporating this labeled amino acid into enzyme substrates or products, researchers can monitor reaction rates and pathways using techniques like mass spectrometry. This helps in characterizing enzyme functions and designing inhibitors for therapeutic use.

Nutritional Studies: In nutritional research, L-Isoleucine-[1-13C] is utilized to study amino acid metabolism and dietary requirements in humans and animals. By tracking the labeled isoleucine in biological samples, scientists can assess protein synthesis rates and amino acid turnover. This contributes to a better understanding of nutritional needs and the development of dietary supplements.