L-Isoleucine-[13C6,15N] is a stable isotope-labeled amino acid that plays a pivotal role in various research and applied bioscience fields. Here are some key applications of L-Isoleucine-[13C6,15N]:
Metabolic Flux Analysis: L-Isoleucine-[13C6,15N] is used in metabolic flux analysis to trace the path of carbon and nitrogen atoms through metabolic networks. By quantifying the incorporation of these isotopes into metabolic intermediates, researchers can map metabolic pathways and understand cellular metabolic dynamics. This is critical for optimizing metabolic engineering strategies and studying disease metabolism.
Proteomics: In proteomics research, L-Isoleucine-[13C6,15N] is utilized to label proteins and peptides in mass spectrometry-based experiments. This helps in the accurate quantification and identification of proteins, improving the understanding of protein expression levels, post-translational modifications, and protein-protein interactions. Such detailed insights are essential for biomarker discovery and developing therapeutic targets.
Nutritional Studies: L-Isoleucine-[13C6,15N] is employed in nutritional studies to investigate amino acid metabolism and utilization in different organisms. By feeding these isotopically labeled amino acids to subjects, researchers can track how efficiently isoleucine is absorbed, metabolized, and incorporated into body tissues. This information aids in designing optimal dietary plans and understanding amino acid requirements.
Enzyme Mechanism Elucidation: This labeled amino acid is also used to study the mechanisms of enzyme-catalyzed reactions involving isoleucine. By incorporating L-Isoleucine-[13C6,15N] into substrates, researchers can observe isotope effects and gain insights into enzyme kinetics and structure-function relationships. This knowledge is crucial for developing enzyme inhibitors and enhancing industrial biocatalysts.
![L-Isoleucine-[13C6,15N]](https://resource.bocsci.com/structure/202468-35-7.gif)
