L-Isoleucine-[13C6]

L-Isoleucine-[13C6] is an isotopically labeled version of the amino acid L-isoleucine used extensively in various scientific applications. Here are some key applications of L-Isoleucine-[13C6]:

Metabolic Flux Analysis: L-Isoleucine-[13C6] is widely utilized in metabolic flux analysis to trace the metabolic pathways in cells. By incorporating this labeled amino acid into cell cultures, researchers can track its incorporation into proteins and other metabolites. This provides insights into the dynamic processes of cellular metabolism and helps in identifying metabolic bottlenecks.

Stable Isotope Labeling: In protein structure studies, L-Isoleucine-[13C6] is used for stable isotope labeling to facilitate the analysis of protein structures using nuclear magnetic resonance (NMR) spectroscopy. The presence of the [13C6] label enhances the resolution and sensitivity of NMR spectra. This technique aids in elucidating the three-dimensional structures of proteins at atomic resolution.

Protein Turnover Studies: L-Isoleucine-[13C6] is employed to study protein turnover rates in various biological systems. By tracking the incorporation and degradation of the labeled amino acid in proteins, researchers can quantify synthesis and degradation rates. This information is essential for understanding protein homeostasis and the regulation of cellular functions.

Nutritional Studies: L-Isoleucine-[13C6] is useful in nutritional research to examine amino acid metabolism and utilization in organisms. By administering the labeled amino acid in dietary studies, scientists can measure its uptake, distribution, and metabolic conversion. This application is vital for understanding dietary requirements and optimizing nutritional formulations.