L-Isoleucine-[d10]

L-Isoleucine-[d10] is an isotopically labeled form of the essential amino acid L-isoleucine often used in various biochemical and biomedical research applications。Here are some key applications of L-Isoleucine-[d10]:

Metabolic Tracing Studies: L-Isoleucine-[d10] is widely used in metabolic tracing experiments to understand metabolic pathways and fluxes。By incorporating this labeled amino acid into organisms or cell cultures researchers can trace its biochemical conversion and integration into various metabolic products。This aids in elucidating the dynamics of amino acid metabolism and identifying potential metabolic disorders

Proteomics Research: In proteomics L-Isoleucine-[d10] serves as an internal standard for mass spectrometry-based quantitative analysis。It helps in the accurate quantification of protein expression levels by providing a reference point for normalizing peptide signals。This is crucial for comparing protein abundance across different samples and identifying biomarkers or therapeutic targets

Pharmacokinetics: L-Isoleucine-[d10] can be employed in the study of pharmacokinetics to track the absorption distribution metabolism and excretion of drugs that interact with amino acids。By monitoring the labeled amino acid in biological samples researchers can gain insights into how drugs affect amino acid metabolism。This information is valuable for optimizing drug dosing and understanding potential side effects

Nutrition Science: In nutrition science L-Isoleucine-[d10] is used to study amino acid requirements and metabolism in humans and animals。Through studies involving isotopically labeled amino acids scientists can determine the rates of protein synthesis and degradation。This knowledge contributes to developing nutritional guidelines and dietary interventions to promote health and manage metabolic diseases