L-Leucine-[1-13C,15N], a stable isotope-labeled amino acid, finds extensive use in biochemical and physiological studies. Here are the key applications of L-Leucine-[1-13C,15N]:
Metabolic Flux Analysis: Delving into metabolic pathways, L-Leucine-[1-13C,15N] serves as a powerful tool for tracing the incorporation and conversion of the labeled isotope in metabolic intermediates. This method sheds light on the dynamics and control of metabolic networks, providing insights into how various metabolic conditions or perturbations influence leucine metabolism.
Protein Turnover Studies: By harnessing L-Leucine-[1-13C,15N], researchers can gauge the rates of protein synthesis and degradation within cells and tissues. The labeled amino acid becomes part of newly formed proteins, allowing for quantification of turnover. This capability is crucial for investigating conditions related to protein balance, such as muscle atrophy and neurodegenerative diseases.
NMR Spectroscopy: Widely employed in NMR spectroscopy, L-Leucine-[1-13C,15N] contributes to determining protein structure and dynamics. The isotope labeling enriches the resolution and sensitivity of NMR signals, facilitating detailed exploration of protein folding, interactions, and conformational changes. This technique plays a pivotal role in structural biology and pharmaceutical development.
Nutritional Studies: In the realm of nutritional research, L-Leucine-[1-13C,15N] serves as a valuable asset for probing amino acid utilization and metabolism in diverse physiological states like fasting, exercise, and disease. By monitoring the labeled leucine, researchers can evaluate how dietary factors and metabolic states impact leucine kinetics, aiding in the formulation of dietary guidelines and enhancing understanding of metabolic wellness.
![L-Leucine-[1-13C,15N]](https://resource.bocsci.com/structure/80134-83-4.gif)
