L-Leucine-[2-13C,15N]

L-Leucine-[2-13C,15N] is a labeled amino acid used as a tracer in various biochemical and metabolic studies. Here are some key applications of L-Leucine-[2-13C,15N]:

Metabolic Flux Analysis: L-Leucine-[2-13C,15N] is employed in metabolic flux analysis to track the pathways and rate at which leucine is metabolized within cells. By tracing the labeled carbon and nitrogen atoms, researchers can map metabolic networks and understand how cells process amino acids. This information is crucial for studying metabolic disorders and optimizing metabolic pathways in biotechnology.

Proteomics: In proteomic studies, L-Leucine-[2-13C,15N] is used for isotope labeling of proteins, enabling mass spectrometry-based quantification of protein synthesis and turnover. The incorporation of the labeled leucine into newly synthesized proteins allows for precise measurement of protein dynamics. This approach is vital for studying protein regulation, identifying biomarkers, and understanding disease mechanisms at the proteomic level.

Nutritional Research: L-Leucine-[2-13C,15N] is valuable in nutritional science to evaluate protein metabolism and nutrient utilization in vivo. By administering labeled leucine to subjects, researchers can monitor its incorporation into body proteins and examine the effects of different diets or nutritional interventions. This application helps in understanding muscle protein synthesis, dietary requirements, and the impact of nutritional supplements.

Cancer Metabolism: In cancer research, L-Leucine-[2-13C,15N] is used to investigate the altered metabolic pathways in tumor cells. By tracing the uptake and utilization of labeled leucine, scientists can uncover specific metabolic adaptations that cancer cells undergo to sustain rapid growth and survival. This information can lead to the development of targeted therapies that disrupt essential metabolic processes in cancer cells.