L-Leucine-[d10,15N]

L-Leucine-[d10,15N] is a stable isotope-labeled amino acid widely utilized in biosciences for its various applications:

Metabolic Flux Analysis: L-Leucine-[d10,15N] is used in metabolic flux analysis to trace amino acid pathways within living organisms. By replacing natural leucine with its labeled variant, researchers can monitor and quantify the incorporation and turnover rates in different metabolic processes. This information is crucial for understanding metabolic dynamics and identifying targets for metabolic engineering.

Protein Turnover Studies: This isotope-labeled leucine is instrumental in studying protein synthesis and degradation rates within cells. By integrating L-Leucine-[d10,15N] into cellular proteins, scientists can measure the rates of new protein production versus protein degradation. Such studies are vital for exploring cellular responses to various stimuli, including stress, nutrition, and disease states.

Quantitative Proteomics: In quantitative proteomics, L-Leucine-[d10,15N] is used as an internal standard to enhance the accuracy and precision of protein quantification through mass spectrometry. The presence of a labeled internal standard enables more precise comparisons between different samples. This application is essential for biomarker discovery and evaluating changes in protein abundance under various biological conditions.

Nutritional Research: L-Leucine-[d10,15N] is valuable for studying amino acid metabolism in nutritional research. By administering this labeled amino acid to subjects, researchers can assess leucine absorption, utilization, and metabolic impacts under different dietary conditions. Such studies provide insights into protein requirements and optimize dietary interventions for health and disease management.