N-BOC-L-Leucine-[d7] H2O

N-BOC-L-Leucine-[d7] H2O, a labeled amino acid derivative, is widely used in bioscience research. Here are some key applications of N-BOC-L-Leucine-[d7] H2O:

Protein Structure Analysis: N-BOC-L-Leucine-[d7] H2O can be utilized in Nuclear Magnetic Resonance (NMR) spectroscopy to investigate protein structures. Its incorporation into proteins allows for precise mapping of leucine residues, aiding in the determination of their 3D structure and dynamics. This is essential for understanding protein folding, function, and interactions at the molecular level.

Metabolomics Studies: In metabolomics, N-BOC-L-Leucine-[d7] H2O serves as an isotopic labeling reagent to track metabolic pathways and fluxes. By incorporating the labeled leucine into metabolic experiments, researchers can trace the incorporation and transformation of this amino acid in various biochemical pathways. This is critical for identifying metabolic alterations associated with diseases and drug treatments.

Peptide Synthesis: N-BOC-L-Leucine-[d7] H2O is employed in the synthesis of deuterated peptides for research purposes. The deuterium labeling aids in mass spectrometry analysis, offering higher resolution and accuracy in identifying and characterizing peptides. This application is particularly useful in studying protein-protein interactions and in developing peptide-based therapeutics.

Drug Metabolism Studies: The labeled amino acid can be used to study the pharmacokinetics and metabolic stability of drug candidates. By incorporating N-BOC-L-Leucine-[d7] H2O into drug molecules, scientists can monitor their metabolic fate and identify potential metabolites. This information is crucial for optimizing drug design and predicting in vivo behavior.