L-Lysine-[1-13C,alpha-15N] Dihydrochloride

L-Lysine-[1-13C,alpha-15N] Dihydrochloride is a vital isotopically labeled amino acid with significant implications in biochemical and physiological research. Here are key applications of L-Lysine-[1-13C,alpha-15N] Dihydrochloride presented with high perplexity and burstiness:

Metabolic Flux Analysis: This specialized compound serves as a cornerstone in metabolic studies, enabling the tracing and quantification of intricate metabolic pathways and fluxes. By integrating L-Lysine-[1-13C,alpha-15N] Dihydrochloride into cultures, researchers embark on a journey to decipher the distribution and conversion of labeled atoms within metabolic processes using cutting-edge mass spectrometry or NMR techniques. This in-depth analysis sheds light on the dynamic nature and regulatory mechanisms of cellular metabolism.

Protein Synthesis Studies: At the forefront of protein research, L-Lysine-[1-13C,alpha-15N] Dihydrochloride plays a pivotal role in unraveling the mysteries of protein synthesis and turnover. The labeling of amino acid residues in nascent proteins enables researchers to meticulously track the incorporation of labeled lysine into polypeptides, providing invaluable insights into protein structure and functionality. This groundbreaking application resonates deeply in the realms of proteomics and structural biology, offering a window into the complex world of protein dynamics.

NMR Spectroscopy: The incorporation of isotopic labels within L-Lysine-[1-13C,alpha-15N] Dihydrochloride revolutionizes NMR spectroscopy of proteins and peptides, elevating the exploration of molecular structures and interactions to new heights. The enriched 13C and 15N atoms generate crisp and detailed spectra, empowering researchers to unravel the intricacies of protein folding dynamics and complex formations with unparalleled clarity. This advanced tool is indispensable for delving into the hidden realms of protein behavior and intermolecular bonds.

Nutritional Studies: In the realm of nutritional exploration, this isotopically labeled lysine emerges as a potent tracer for investigating amino acid metabolism within organisms. By administering L-Lysine-[1-13C,alpha-15N] Dihydrochloride, researchers embark on a quest to track the absorption, utilization, and excretion patterns of lysine, unraveling the intricate web of amino acid dynamics. These studies play a pivotal role in deciphering nutritional requirements and optimizing dietary formulations, paving the way for a deeper understanding of organismal sustenance and metabolic balance.