L-Lysine-[13C6,15N2,d9] monohydrochloride

L-Lysine-[13C6,15N2,d9] monohydrochloride, a labeled amino acid utilized predominantly in scientific research, serves as a versatile tool in tracing and metabolic studies. Here are key applications of L-Lysine-[13C6,15N2,d9] monohydrochloride presented with high perplexity and burstiness:

Metabolic Flux Analysis: Engaging in metabolic experiments, researchers employ L-Lysine-[13C6,15N2,d9] to unravel the intricate pathways and dynamic shifts within amino acid metabolism at the cellular level. By integrating this labeled lysine into metabolic studies, scientists can meticulously monitor its assimilation and turnover rates, shedding light on the complex web of metabolic fluxes. This methodological approach is pivotal in deciphering nutrient utilization and optimizing metabolic pathways.

Protein Synthesis Studies: Within the realm of proteomics, L-Lysine-[13C6,15N2,d9] emerges as a key component for in vivo protein labeling, paving the way for subsequent mass spectrometric analysis. This strategic labeling strategy enables precise quantification and detailed analysis of protein expression and synthesis rates across diverse experimental conditions. Through such investigations, profound insights into protein turnover dynamics and regulatory mechanisms are gleaned, propelling the field of functional genomics forward.

Enzyme Mechanism Investigation: By incorporating isotopic labels like L-Lysine-[13C6,15N2,d9], researchers gain unparalleled insights into the intricate mechanisms and transient states of enzymes. Substituting natural lysine with its labeled counterpart in enzyme assays allows for in-depth exploration of enzyme kinetics and specific catalytic steps. This rigorous approach aids in uncovering novel enzyme functionalities and enhancing the design of potent inhibitors, pushing the boundaries of enzyme research to new horizons.

Nutritional Biochemistry: Positioned at the intersection of nutritional science and biochemistry, L-Lysine-[13C6,15N2,d9] emerges as a pivotal tool for elucidating the metabolic fate of lysine, an indispensable amino acid. Scientists leverage this compound to probe how dietary lysine is absorbed, metabolized, and utilized across various physiological scenarios. These comprehensive studies contribute significantly to our understanding of amino acid dynamics and nutritional requirements, offering valuable insights to the field of nutrition science.