L-Methionine-[1,2,3,4-13C4,15N]

L-Methionine-[1,2,3,4-13C4,15N], a stable isotope-labeled compound, finds diverse applications in biochemical and molecular domains. Here are the key applications of L-Methionine-[1,2,3,4-13C4,15N]:

Metabolic Flux Analysis: Crucial for unraveling metabolic intricacies, L-Methionine-[1,2,3,4-13C4,15N] serves as a cornerstone in studying cellular fluxes. By integrating this labeled amino acid into metabolic pathways, researchers can meticulously trace the movement of methyl groups and other intermediates. This comprehensive approach aids in deciphering cellular metabolism nuances, thereby enhancing our grasp of industrial bioprocess optimization.

Protein Turnover Studies: Aiding in the precise evaluation of protein dynamics, stable isotope labeling with L-Methionine-[1,2,3,4-13C4,15N] enables the accurate measurement of protein turnover rates. In the realm of mass spectrometry-based proteomics, it facilitates the real-time tracking and quantification of newly synthesized proteins. This application stands pivotal in probing cellular responses and unraveling the intricate dance of protein synthesis and degradation.

Methylation Studies: Delving into the realm of methylation processes, L-Methionine-[1,2,3,4-13C4,15N] emerges as a crucial tool in DNA and protein research. By enriching the cellular milieu with this labeled amino acid, researchers can meticulously monitor methyl group transfers during methylation reactions. This insightful approach sheds light on epigenetic regulations and unveils the profound role of methylation in various disease pathways, expanding our understanding of molecular mechanisms.

Nutritional Research: In the domain of nutritional exploration, this labeled compound plays a pivotal role in dissecting the metabolism and utilization of methionine within the human body. By diligently tracking the labeled methionine, scientists gain profound insights into amino acid catabolism and the intricate interplay of methionine in maintaining health and combating diseases. Such investigations pave the way for informed dietary recommendations and the development of strategic therapeutic interventions.