L-Methionine-[d8,15N]

L-Methionine-[d8,15N] is an isotopically labeled amino acid valuable in various research applications due to its stable isotopic composition. Here are some key applications of L-Methionine-[d8,15N]:

Metabolic Flux Analysis: L-Methionine-[d8,15N] is used in metabolic flux analysis to trace the pathways of methionine metabolism in cells. By incorporating this labeled amino acid into metabolic studies, researchers can quantify the rates of metabolic reactions and understand the dynamics of methionine utilization. This provides crucial insights into cellular metabolism and can help identify metabolic dysfunctions in disease states.

Protein Turnover Studies: In protein biology, L-Methionine-[d8,15N] is utilized to investigate protein synthesis and degradation rates within cells. By tracking the incorporation and turnover of labeled methionine in proteins, scientists can measure protein half-lives and understand the dynamics of cellular proteostasis. These studies are important for elucidating the roles of specific proteins in health and disease.

Mass Spectrometry: L-Methionine-[d8,15N] serves as a valuable tool in mass spectrometry-based proteomics. The incorporation of this isotopically labeled methionine allows for precise quantification and identification of peptides in complex biological samples. This approach is essential for studying protein expression, post-translational modifications, and protein-protein interactions.

Nutritional Biochemistry: In nutritional studies, L-Methionine-[d8,15N] is used to understand methionine metabolism and its role in health and disease. By administering the labeled amino acid and monitoring its metabolic fate, researchers can investigate the impact of dietary methionine on metabolic pathways and overall organismal health. This knowledge is critical for developing dietary interventions and therapeutic strategies for metabolic disorders.