L-Phenylalanine-[13C9]

L-Phenylalanine-[13C9] is a stable isotope-labeled amino acid widely used in various scientific fields. Here are some key applications of L-Phenylalanine-[13C9].

Metabolic Flux Analysis: L-Phenylalanine-[13C9] is used to trace metabolic pathways and measure metabolic fluxes in cellular systems. By incorporating this labeled amino acid into cells, researchers can use mass spectrometry to track its conversion into other metabolic products. This provides detailed insights into protein synthesis, amino acid metabolism, and metabolic regulation.

Protein Turnover Studies: In proteomics, L-Phenylalanine-[13C9] helps quantify protein dynamics and turnover rates within an organism. Researchers can distinguish between newly synthesized and existing proteins by integrating the labeled amino acid into the diet of experimental models. This information is vital for understanding cellular protein homeostasis and the effects of disease or treatment interventions on protein metabolism.

Diagnostic Imaging: L-Phenylalanine-[13C9] can be utilized in positron emission tomography (PET) imaging to study brain function and diagnose neurological conditions. By combining the labeled amino acid with radiotracers, clinicians can observe its uptake and distribution in the brain. This technique is particularly useful in diagnosing and monitoring disorders such as brain tumors and phenylketonuria.

Pharmacokinetics Studies: Researchers employ L-Phenylalanine-[13C9] to investigate the pharmacokinetics of amino acid-based drugs and therapies. By tracking the labeled compound through various biological systems, scientists can determine absorption, distribution, metabolism, and excretion profiles. This aids in optimizing dosing strategies and understanding the bioavailability of therapeutics.