L-Phenylalanine-[d8,15N]

L-Phenylalanine-[d8,15N] is an isotopically labeled amino acid that serves as a powerful tool in various research and industrial applications. Here are some key applications of L-Phenylalanine-[d8,15N].

Metabolic Flux Analysis: L-Phenylalanine-[d8,15N] is widely used in metabolic studies to trace the flow of carbon and nitrogen through metabolic pathways. By incorporating this labeled amino acid into cells or organisms, researchers can monitor its integration and subsequent metabolic transformations using mass spectrometry or NMR. This allows for a detailed understanding of metabolic networks and the identification of bottlenecks in metabolic processes.

Protein Structure Elucidation: In structural biology, L-Phenylalanine-[d8,15N] is used in NMR spectroscopy to study protein folding and interactions. The deuterium and nitrogen-15 labels provide unique spectral signatures that facilitate the analysis of protein dynamics and conformation. This can be particularly useful for elucidating the structure of complex proteins or protein-ligand interactions.

Pharmacokinetic Studies: L-Phenylalanine-[d8,15N] is employed in pharmacokinetic experiments to track the absorption, distribution, metabolism, and excretion of drugs. By incorporating the labeled amino acid into pharmaceutical compounds, researchers can follow the compound's behavior in vivo using advanced analytical techniques. This helps in optimizing drug formulations and understanding drug metabolism.

Nutritional Research: In nutritional science, L-Phenylalanine-[d8,15N] is used to study amino acid metabolism and protein turnover in humans and animals. By administering the labeled amino acid and measuring its incorporation into proteins and other metabolites, researchers can gain insights into dietary protein utilization and metabolic health. This information is crucial for developing effective dietary recommendations and understanding metabolic disorders.