L-Proline-[1-13C,15N]

L-Proline-[1-13C,15N] is an isotopically labeled amino acid used in various biotechnological and research applications. Here are some key applications of L-Proline-[1-13C,15N]:

Metabolic Flux Analysis: L-Proline-[1-13C,15N] is used in metabolic flux analysis to track the flow of carbon and nitrogen through metabolic pathways. By incorporating this labeled amino acid into metabolic studies, researchers can quantify and map complex metabolic networks. This is essential for understanding cellular metabolism and optimizing biotechnological processes.

Protein Structure Determination: In structural biology, L-Proline-[1-13C,15N] is employed in nuclear magnetic resonance (NMR) spectroscopy to study protein structures. The isotopic labeling allows for specific insights into the conformational dynamics and spatial arrangement of proteins. This helps in uncovering the structural basis of protein function and interactions.

Enzyme Mechanism Studies: Researchers use L-Proline-[1-13C,15N] to investigate enzyme mechanisms and substrate interactions. The isotopic labels provide detailed information on the enzyme’s active site and the chemical changes occurring during catalysis. This knowledge is crucial for enzyme engineering and the development of enzyme inhibitors.

Drug Development: L-Proline-[1-13C,15N] is valuable in drug discovery research for studying the pharmacokinetics and dynamics of proline-containing compounds. By tracing the labeled amino acid, scientists can analyze the absorption, distribution, metabolism, and excretion (ADME) of potential drugs. This information is vital for optimizing the efficacy and safety profiles of new therapeutics.