L-Proline-[13C5]

L-Proline-[13C5] is an isotopically labeled amino acid, commonly used in various biochemical and biotechnological applications. Here are some key applications of L-Proline-[13C5]:

Metabolic Flux Analysis: L-Proline-[13C5] is employed in metabolic flux analysis to trace the pathways and rates of metabolic reactions within cells. By incorporating the labeled proline into cellular systems, researchers can track its distribution and conversion into other metabolites. This helps in understanding cellular metabolism and identifying potential metabolic bottlenecks.

Protein Structure Elucidation: L-Proline-[13C5] is valuable in protein NMR spectroscopy for determining protein structures. The carbon-13 labeling of proline provides specific NMR signals that help in mapping out the three-dimensional structure of proteins. This is crucial for understanding protein function and for drug design efforts targeting specific proteins.

Stable Isotope Labeling: In stable isotope labeling studies, L-Proline-[13C5] is used to label proteins or peptides in quantitative proteomics. By providing a distinct isotopic signature, it allows for precise measurement of protein abundance and dynamics in complex biological samples. This facilitates the study of proteome changes under different physiological or pathological conditions.

Biosynthetic Pathway Investigation: L-Proline-[13C5] can be used to investigate the biosynthetic pathways of proline and its derivatives in various organisms. By feeding the labeled proline to cells or tissues, researchers can trace its incorporation and transformation through different biosynthetic routes. This is essential for understanding the regulation and engineering of biosynthetic pathways for biotechnological applications.