L-Serine-[2,3,3-d3,15N]

L-Serine-[2,3,3-d3,15N], an isotopically labeled form of L-serine enriched with deuterium and nitrogen-15, finds diverse applications in scientific research. Here are the key applications of L-Serine-[2,3,3-d3,15N]:

Metabolic Flux Analysis: Widely utilized in metabolic flux analysis, L-Serine-[2,3,3-d3,15N] serves as a valuable tool for tracing the intricate metabolic pathways involving serine. By meticulously tracking the movements of the labeled atoms across various metabolic intermediates, researchers can unravel the flux of metabolites and pinpoint pivotal regulatory points. This application plays a vital role in comprehending the intricacies of cell metabolism and optimizing metabolic networks.

Protein Structure Studies: The strategic isotopic labeling of L-serine with deuterium and nitrogen-15 offers a window into the world of protein structures through NMR spectroscopy. By incorporating L-Serine-[2,3,3-d3,15N] into proteins, scientists gain access to detailed high-resolution structural insights into the amino acid environment. Such information is indispensable for deciphering the dynamic processes of protein folding and intricate interactions within the molecular realm.

Stable Isotope Labeling in Peptide Synthesis: In the realm of mass spectrometry analysis, L-Serine-[2,3,3-d3,15N] plays a crucial role in the synthesis of peptides, introducing stable isotopes for precision measurements. These labeled peptides act as internal benchmarks, facilitating the accurate quantification of target peptides within complex biological samples. This application holds particular significance in proteomics and the quest for biomarker discovery, advancing our understanding of biological systems at a molecular level.

Functional Genomics: Delving into gene functions and regulatory mechanisms linked to serine metabolism, L-Serine-[2,3,3-d3,15N] emerges as a versatile tool in scientific inquiry. By monitoring the incorporation and turnover of this labeled amino acid across different genetic contexts and environmental conditions, researchers can infer the roles of specific genes in serine biosynthesis and utilization. This knowledge forms the linchpin for unraveling the genetic underpinnings of metabolic disorders and shaping the landscape of therapeutic interventions with precision.