L-Threonine-[13C4,15N]

L-Threonine-[13C4,15N], an isotopically labeled amino acid, finds diverse applications in bioscience. Here are the key applications of L-Threonine-[13C4,15N], depicted with high perplexity and burstiness:

Metabolic Flux Analysis: Delving into metabolic flux analysis, scientists utilize L-Threonine-[13C4,15N] to trace the intricate pathways and rates of metabolic reactions occurring within cellular systems. By integrating this labeled amino acid into metabolic networks, researchers can meticulously track its dispersion and transformation, unraveling profound insights into cell metabolism. This technique serves as a cornerstone for comprehending cellular physiology and fine-tuning metabolic engineering strategies.

Protein Structure and Function Studies: In the realm of protein research, L-Threonine-[13C4,15N] emerges as a vital tool for probing protein structure and function through the lens of NMR spectroscopy and mass spectrometry. Acting as a distinctive marker, this labeled amino acid enables scientists to monitor its assimilation into proteins, unveiling alterations in protein dynamics and interactions. This critical data plays a pivotal role in deciphering protein mechanisms and crafting innovative therapeutic approaches.

Nutritional Studies: Within the domain of nutritional research, L-Threonine-[13C4,15N] serves as a key player in unraveling the mysteries of amino acid metabolism and utilization within organisms. By administering this isotopically labeled amino acid to animals or cells, researchers can quantify its absorption, integration into tissues, and excretion. These investigations contribute significantly to understanding the nutritional necessities and health implications of amino acids present in various dietary regimens.

Biosynthetic Pathway Elucidation: At the forefront of biosynthetic pathway exploration, L-Threonine-[13C4,15N] takes center stage in mapping out the intricate synthesis and breakdown of threonine and its associated compounds. This isotopically labeled amino acid empowers researchers to chart the biochemical pathways and intermediate stages involved in threonine metabolism, shedding light on the biotechnological applications, including the production of threonine and its derivatives in microbial systems.