L-Threonine-[13C4]

L-Threonine-[13C4], an isotopically labeled amino acid, utilized in diverse research contexts to monitor metabolic processes and investigate protein synthesis, finds application across multiple domains. Here are the key applications of L-Threonine-[13C4]:

Metabolic Flux Analysis: Embedded within the realm of metabolic flux analysis, L-Threonine-[13C4] plays a pivotal role in delineating the intricate pathways of carbon flow within cellular metabolism. By integrating this labeled amino acid into metabolic processes, researchers can meticulously track specific pathways like protein synthesis and degradation. This approach not only illuminates the dynamic nuances of metabolism but also unveils bottlenecks impeding bioprocesses.

Proteomics Research: At the crux of proteomics research, L-Threonine-[13C4] emerges as a potent instrument for quantitative mass spectrometry. It facilitates precise quantification of protein synthesis rates and turnover by tracing the integration of the labeled amino acid into nascent proteins. This application proves indispensable in examining cellular responses to diverse stressors and treatments at the protein level, offering profound insights into cellular dynamics.

Stable Isotope Labeling in Mammals (SILAM): Within the domain of SILAM, L-Threonine-[13C4] serves as a linchpin for studying protein dynamics and metabolic shifts in animal models. By supplementing animal diets with labeled threonine, researchers can dissect tissue-specific protein turnover and metabolic alterations. This methodology sheds light on disease trajectory and the impacts of therapeutic interventions, unveiling critical insights into physiological adaptations.

Nutritional Studies: In the realm of nutritional sciences, L-Threonine-[13C4] stands as a cornerstone for exploring amino acid metabolism and nutrient utilization. Leveraging this labeled compound, researchers delve into the absorption, metabolism, and integration of dietary threonine into proteins. This empirical data is crucial for optimizing animal and human diets, ensuring a harmonious balance of nutrition and fostering robust health outcomes.