L-Tyrosine-[2,3-13C2,15N]

L-Tyrosine-[2,3-13C2,15N], an amino acid enriched with stable isotopes, finds diverse applications in scientific research. Here are the key applications of L-Tyrosine-[2,3-13C2,15N]

Metabolic Flux Analysis: This specialized form of L-Tyrosine is harnessed in metabolic flux analysis to monitor the incorporation and metamorphosis of tyrosine across metabolic pathways. Using techniques such as mass spectrometry or NMR, researchers can meticulously measure the metabolic fluxes and unravel the dynamic behaviors of pathways in cells and tissues. This intricate data plays a pivotal role in fueling advancements in metabolic engineering and in unraveling the mysteries of disease metabolism

Protein and Peptide Labeling: By embedding L-Tyrosine-[2,3-13C2,15N] within proteins and peptides, researchers can conduct meticulous examinations of protein structure and functionality using methods such as NMR spectroscopy. The isotopic tagging amplifies the sensitivity and resolution of NMR signals, facilitating intricate analyses of structural nuances and dynamic movements within proteins. This methodology is particularly invaluable for deciphering protein folding processes, interactions, and conformational transitions

Kinetic Studies: L-Tyrosine-[2,3-13C2,15N] serves as a critical component in kinetic isotope effect studies aimed at probing reaction mechanisms. Through comparisons of reaction rates between labeled and non-labeled tyrosine, researchers glean valuable insights into transition states and reaction pathways. This knowledge proves indispensable in elucidating enzyme mechanisms and in strategizing the development of potent inhibitors, leading to breakthroughs in therapeutic interventions

Neuroscience Research: In the realm of neuroscience research, L-Tyrosine-[2,3-13C2,15N] stands as a vital tool for tracing and quantifying the synthesis of neurotransmitters like dopamine and norepinephrine. By monitoring the metabolization of labeled tyrosine, scientists delve into the intricate world of neurotransmitter metabolism and its variations in diverse neurological conditions. This application plays a pivotal role in enhancing our understanding of brain functionality and in crafting innovative treatments for neuropsychiatric disorders