L-Tyrosine-[d7,15N]

L-Tyrosine-[d7,15N] is a stable isotope-labeled amino acid useful in a variety of scientific applications. Here are some key applications of L-Tyrosine-[d7,15N]:

Metabolic Flux Analysis: L-Tyrosine-[d7,15N] is used in metabolic flux analysis to trace the pathways and fluxes of amino acid metabolism. By incorporating this labeled amino acid into cells or organisms, researchers can track its conversion into other metabolites. This provides detailed insights into metabolic networks and enzyme functions.

Protein Turnover Studies: In proteomics, L-Tyrosine-[d7,15N] serves as a tracer for studying protein synthesis and degradation rates. By detecting the incorporation of the labeled tyrosine into newly synthesized proteins, scientists can quantify protein turnover. This is crucial for understanding cellular protein dynamics and responses to various stimuli.

Mass Spectrometry: L-Tyrosine-[d7,15N] is a valuable internal standard in mass spectrometry-based quantitative proteomics. The isotope-labeled tyrosine helps in accurately quantifying proteins in complex samples by providing a reference point. This enhances the precision and reliability of proteomic analyses.

Neurotransmitter Studies: L-Tyrosine-[d7,15N] is used to study the biosynthesis of catecholamine neurotransmitters, such as dopamine and norepinephrine. By tracing the incorporation of the labeled tyrosine into these neurotransmitters, researchers can investigate the regulatory mechanisms of neurotransmitter production. This information is vital for understanding neurological functions and disorders.