L-Tyrosine-[phenol-4-13C]

L-Tyrosine-[phenol-4-13C], an isotopically labeled amino acid, is widely employed in diverse biochemical and physiological research endeavors. Here are the key applications of L-Tyrosine-[phenol-4-13C]:

Metabolic Flux Analysis: As a crucial tracer, L-Tyrosine-[phenol-4-13C] plays a vital role in exploring metabolic pathways and fluxes. By integrating this isotopically labeled amino acid into metabolic processes, researchers can intricately trace the carbon atom flow across various metabolic routes. This method sheds light on enzyme functions, regulatory mechanisms, and the complex dynamics governing cellular metabolism.

Protein Structure and Dynamics: In the field of protein biochemistry, L-Tyrosine-[phenol-4-13C] is invaluable in nuclear magnetic resonance (NMR) spectroscopy for probing protein structure and dynamics. The precise insertion of the 13C isotope within the tyrosine residue enables in-depth structural analysis. Researchers leverage this technique to gain insights into protein folding, conformational alterations, and protein-ligand interactions, unraveling the intricate behaviors of proteins.

Stable Isotope Labeling: Serving as a cornerstone in stable isotope labeling experiments, L-Tyrosine-[phenol-4-13C] facilitates quantitative analysis in diverse domains. By incorporating this labeled amino acid into biological samples, researchers can quantify the rates of tyrosine incorporation and turnover in proteins and other biomolecules. This methodology is indispensable for investigations across proteomics, metabolic engineering, and pharmacokinetics, offering a comprehensive understanding of biological processes.

Neurotransmitter Research: Delving into neurotransmitter biosynthesis and metabolism, L-Tyrosine-[phenol-4-13C] emerges as a valuable tool. Tyrosine acts as a precursor to key neurotransmitters like dopamine, norepinephrine, and epinephrine. Utilizing the 13C-labeled variant allows researchers to trace the biochemical pathways leading to neurotransmitter synthesis. This approach provides crucial insights into neurobiology and the management of neurological disorders, enhancing our understanding of brain function and therapeutic interventions.