L-Valine-[1-13C,15N]

L-Valine-[1-13C,15N] is a stable isotope-labeled amino acid used in various scientific research applications. Here are some key applications for L-Valine-[1-13C,15N]:

Metabolic Flux Analysis: L-Valine-[1-13C,15N] is employed to study metabolic fluxes by tracking the incorporation of the labeled carbon and nitrogen atoms into metabolic pathways. This helps researchers understand the dynamics of amino acid metabolism and its role in cellular processes. It provides precise data essential for metabolic engineering and the development of therapeutic strategies.

Protein Structure and Dynamics: In structural biology, L-Valine-[1-13C,15N] is used in NMR spectroscopy to investigate protein folding, stability, and conformational changes. The isotope labels provide distinct signals that offer detailed information on the protein’s backbone and side-chain interactions. This application is crucial for elucidating the molecular mechanisms underlying protein function and dysfunction.

Nutritional Studies: L-Valine-[1-13C,15N] is applied in nutrition research to assess the metabolism and utilization of valine in different physiological states. By tracking the isotope through metabolic processes, researchers can determine the bioavailability and nutritional impact of valine in diets. This knowledge helps inform dietary recommendations and the development of nutritional supplements.

Functional Genomics: In functional genomics, L-Valine-[1-13C,15N] can be utilized to study the role of valine in gene expression and regulation. By integrating the labeled amino acid into experimental models, scientists can monitor how valine influences transcriptional and translational processes. This application aids in understanding the genetic basis of metabolic regulation and related diseases.