L-Valine-[3-d]

L-Valine-[2-13C] is a stable isotope-labeled amino acid used in various scientific applications. Here are some key applications of L-Valine-[2-13C]:

Metabolic Flux Analysis: L-Valine-[2-13C] is crucial for studying metabolic pathways and understanding how cells utilize nutrients. By incorporating this labeled amino acid into metabolic flux analysis experiments, researchers can trace the flow of carbon through metabolic cycles. This enables precise quantification of metabolic rates and the identification of pathway bottlenecks in different organisms.

Protein Structure and Dynamics: In structural biology, L-Valine-[2-13C] is used in NMR spectroscopy to study protein structures and dynamics. The 13C label provides a unique signal that helps elucidate the spatial arrangement and movement of specific amino acid residues within proteins. This information is essential for understanding protein function, interactions, and stability.

Nutritional Studies: L-Valine-[2-13C] can be employed in nutritional research to investigate the metabolism and utilization of amino acids in humans and animals. By administering this labeled compound and tracking its absorption and incorporation into tissues, scientists can assess protein synthesis rates and the effects of different diets. This helps in developing optimized dietary recommendations and supplements.

Agricultural Biotechnology: In plant science, L-Valine-[2-13C] is used to study amino acid metabolism and nitrogen utilization in crops. By feeding plants with this labeled amino acid, researchers can monitor how plants incorporate and redistribute nitrogen. This knowledge assists in breeding and engineering crops for improved nitrogen use efficiency, contributing to sustainable agricultural practices.