L-Valine-[dimethyl-13C2]

L-Valine-[dimethyl-13C2], an isotopically labeled essential amino acid, finds diverse applications in research. Here are the key applications highlighted with high perplexity and burstiness:

Metabolic Flux Analysis: Delving into metabolic flux analysis, L-Valine-[dimethyl-13C2] serves as a crucial tool for tracing metabolic pathways within living cells. By monitoring the incorporation of 13C into various metabolites, researchers can precisely quantify the flow of metabolic intermediates. This technique is indispensable for gaining insights into cellular metabolism intricacies and refining biotechnological processes to boost efficiency.

Stable Isotope Labeling in Proteomics: In the realm of proteomics, L-Valine-[dimethyl-13C2] plays a pivotal role in Stable Isotope Labeling by Amino acids in Cell culture (SILAC). This technique facilitates the quantitative assessment of protein expression disparities across different samples, enabling the identification of key biomarkers, decoding protein functions, and exploring disease mechanisms at a deeper protein level perspective.

Protein Structure Determination: Enabling NMR (Nuclear Magnetic Resonance) spectroscopy studies, L-Valine-[dimethyl-13C2] contributes significantly to unraveling protein structures. The integration of 13C isotopes heightens the resolution of NMR spectra, furnishing detailed insights into protein folding dynamics and intricate interactions. This application holds immense value in drug design endeavors and enhancing comprehension of protein-ligand binding phenomena.

Agricultural Research: In the domain of agricultural research, L-Valine-[dimethyl-13C2] is a key player in plant metabolic investigations focused on amino acid biosynthesis and transport mechanisms. By tracking the journey of labeled L-Valine, researchers gain a profound understanding of how plants synthesize and distribute this essential amino acid. This knowledge is instrumental in the development of crops with superior nutrient profiles and heightened resilience to environmental stresses, bolstering agricultural sustainability.