Methyl α-D-glucopyranoside-[1-13C]

Methyl α-D-glucopyranoside-[1-13C], a stable isotope-labeled compound, finds diverse applications in metabolic and biochemical research. Here are the key applications:

Metabolic Flux Analysis: This compound serves as a crucial tool in metabolic flux analysis, enabling researchers to trace the intricate pathways of glucose metabolism. By introducing the [1-13C] label, scientists can meticulously track the flow of the labeled carbon atom through various metabolic pathways. This methodological approach provides invaluable insights into cellular metabolism and sheds light on pivotal regulatory steps governing metabolic processes.

NMR Spectroscopy: Leveraging Methyl α-D-glucopyranoside-[1-13C] in nuclear magnetic resonance (NMR) spectroscopy offers a window into the structural and dynamic characteristics of carbohydrates. The incorporation of the 13C label enhances sensitivity and resolution in NMR studies, empowering researchers to delve deep into molecular conformations and interactions. This advanced technique furnishes researchers with intricate details about carbohydrate structures, paving the way for a comprehensive understanding of molecular dynamics.

Biochemical Pathway Elucidation: This isotope-labeled compound plays a critical role in unraveling intricate biochemical pathways associated with glucose metabolism. By tracing the labeled carbon atom, scientists can uncover the specific enzymes and intermediates participating in glucose metabolic pathways. This knowledge is paramount for dissecting disease mechanisms at the molecular level and shaping the development of precise, targeted therapeutic interventions.

Glycoprotein Biosynthesis: Exploring the biosynthesis of glycoproteins and glycoconjugates, Methyl α-D-glucopyranoside-[1-13C] offers a powerful tool for studying complex biological processes. The incorporation of the labeled compound enables researchers to map out glycan structures and trace their origins within metabolic pathways. This application holds significance in advancing research in cell biology and enhancing the production of therapeutic glycoproteins, unlocking new realms of understanding in the field of glycoprotein biosynthesis.