N-Acetyl-Asp-Glu-OH-[15N2]

N-Acetyl-Asp-Glu-OH-[15N2] is a stable isotope-labeled dipeptide that has various applications in scientific research, particularly in protein and peptide studies. Here are some key applications of N-Acetyl-Asp-Glu-OH-[15N2]:

Metabolomics Research: In metabolomics, N-Acetyl-Asp-Glu-OH-[15N2] can be utilized as an internal standard for quantitative analysis of metabolic compounds. By incorporating this isotope-labeled dipeptide into samples, researchers can achieve accurate quantification of metabolites through mass spectrometry. This is essential for studying metabolic pathways and understanding disease-related metabolic alterations.

Protein Structure Analysis: This isotope-labeled peptide can be used in nuclear magnetic resonance (NMR) spectroscopy to investigate protein folding and dynamics. The [15N2] labeling allows for enhanced sensitivity in NMR experiments, facilitating detailed structural analysis of peptides and proteins. Such studies are crucial for elucidating mechanisms of protein interactions and conformational changes.

Biopharmaceutical Development: N-Acetyl-Asp-Glu-OH-[15N2] can serve as a reference material in the development and quality control of peptide-based therapeutics. By providing a well-characterized standard, it assists in verifying the identity and purity of peptides in pharmaceutical formulations. This ensures compliance with regulatory standards and maintains consistency in drug production.

Enzymatic Reaction Studies: Researchers can use this labeled dipeptide to study enzymatic reactions involving aspartate and glutamate residues. The [15N2] label allows for tracing the fate of nitrogen atoms during enzymatic processes such as transamination and amidation. Insights gained from these studies can contribute to a deeper understanding of enzyme specificity and catalytic mechanisms.