N-Acetyl-L-methionine-[15N]

N-Acetyl-L-methionine-[15N], an isotopically labeled amino acid, serves as a unique tool with diverse scientific applications. Here are key applications of N-Acetyl-L-methionine-[15N] presented with high perplexity and burstiness:

Metabolic Tracing Studies: Delving into intricate metabolic pathways, researchers utilize this compound in metabolic tracing studies to unravel the intricacies of methionine metabolism. By introducing the [15N] label, scientists can meticulously track the assimilation and conversion of methionine within cells and tissues. Such investigations offer valuable insights into metabolic disorders and shed light on the pivotal role of methionine in disease progression.

Protein Labeling: At the forefront of structural biology, N-Acetyl-L-methionine-[15N] finds application in protein labeling for nuclear magnetic resonance (NMR) and mass spectrometry analyses. The incorporation of the [15N] isotope facilitates detailed exploration of protein structure and function at an atomic level. This methodological approach enhances understanding of protein dynamics and interactions, enriching the study of protein behavior with nuanced insights.

Nutritional Studies: In the realm of nutritional science, researchers harness N-Acetyl-L-methionine-[15N] to delve into protein turnover and amino acid utilization. By monitoring the [15N] label, scientists can analyze the rates of protein synthesis and degradation under varying dietary conditions. These observations contribute to the development of targeted nutritional interventions aimed at enhancing health outcomes and managing metabolic disorders through a deep understanding of amino acid metabolism.

Enzyme Kinetics: Leveraging the labeled N-Acetyl-L-methionine, researchers embark on explorations of enzyme kinetics, focusing on enzymes involved in methionine-related processes. By employing this compound, scientists can monitor enzyme activity and gain insights into catalytic mechanisms. This knowledge plays a crucial role in enzyme engineering and facilitates the development of enzyme inhibitors for therapeutic applications, offering a deeper understanding of enzymatic processes and their potential pharmaceutical implications.