Sirolimus-[13C,d3]

Sirolimus-[13C,d3], a stable isotope-labeled compound, is a versatile research tool utilized across various scientific fields, particularly in pharmacology and biochemistry. Here are key applications of Sirolimus-[13C,d3] presented with high perplexity and burstiness:

Pharmacokinetics Studies: By leveraging Sirolimus-[13C,d3] in pharmacokinetic investigations, researchers delve deep into the absorption, distribution, metabolism, and excretion (ADME) of sirolimus, an immunosuppressive agent. This labeled compound enables precise tracking of the drug's metabolic pathways and interactions within the body.

Drug Metabolism Research: In the realm of drug metabolism, Sirolimus-[13C,d3] emerges as a cornerstone reference compound, shedding light on the intricate biotransformation processes of sirolimus. It facilitates the identification and quantification of metabolites, evaluation of enzyme participation, and assessment of sirolimus' metabolic stability. Such data is indispensable for driving drug development endeavors and anticipating potential drug-drug interactions, reshaping the landscape of pharmaceutical research.

Proteomics: Sirolimus-[13C,d3] unfolds its potential in quantitative proteomics, unraveling the complex web of protein-drug interactions and signaling cascades. By integrating this labeled compound, researchers delve into stable isotope labeling by amino acids in cell culture (SILAC) experiments, unearthing profound insights into protein expression variations and regulatory mechanisms influenced by sirolimus treatment. This sophisticated technique revolutionizes our understanding of cellular processes and drug-protein dynamics, paving the way for novel therapeutic strategies.

Analytical Chemistry: Harnessing Sirolimus-[13C,d3] as an internal standard in mass spectrometry-based assays propels analytical chemistry to new heights, ensuring the precision and accuracy of quantitative sirolimus measurements in biological specimens.