β,β-trehalose-[UL-13C12]

β,β-Trehalose-[UL-13C12], a trehalose molecule uniformly labeled with 12 carbon-13 atoms, is a versatile tool widely employed in biosciences for its tracing and analytical capabilities. Here are the key applications of β,β-trehalose-[UL-13C12]:

Metabolic Flux Analysis: Pioneering metabolic flux analysis (MFA) leverages β,β-trehalose-[UL-13C12] to delve into the intricate pathways of carbohydrate metabolism within cells. By meticulously tracing the labeled carbon atoms, researchers gain insights into the intricate distribution and utilization of trehalose across diverse metabolic routes. This approach sheds light on the dynamic nature of metabolism, illuminating the regulatory mechanisms governing energy storage and expenditure.

Proteomics Research: In the realm of proteomics, β,β-trehalose-[UL-13C12] emerges as a key player in unraveling the mysteries of protein glycosylation. Acting as a labeled sugar source, it offers a window into the incorporation of sugars into glycoproteins, unveiling crucial details about protein structure, function, and glycosylation patterns. This tool proves invaluable in exploring protein folding stability and deciphering the intricacies of cellular signaling pathways.

Plant Physiology: Delving into the realm of plant physiology, β,β-trehalose-[UL-13C12] serves as a beacon of insight into the role of trehalose in plant stress responses and developmental processes. By harnessing labeled trehalose, scientists embark on a journey to unveil the synthesis, transport, and metabolism of this compound in plants under varying environmental conditions. This undertaking sheds light on how plants navigate abiotic stresses like drought and salinity, enriching our understanding of plant resilience mechanisms.

Biotechnology: In the realm of biotechnology, the applications of β,β-trehalose-[UL-13C12] are multifaceted, enhancing the production of recombinant proteins and other bioproducts. By providing stability to enzymes and cells during bioprocessing, it stands as a cornerstone of innovation in biotechnological processes. Its protective properties against stress conditions such as freeze-drying and high osmolarity make it a prized asset in fermentation processes and the formulation of biopharmaceuticals. This utilization augments the yield and quality of biotechnological products, driving advancements in the field.