Xanthosine-[13C5]

Xanthosine-[13C5] is a carbon-labeled nucleoside analog used in various scientific and research applications. Here are some key applications of Xanthosine-[13C5]:

Metabolic Flux Analysis: Xanthosine-[13C5] is utilized in metabolic flux analysis to trace the flow of carbon through nucleotide metabolism pathways. By incorporating this labeled compound, researchers can track the conversion processes of nucleotides in cells. This application aids in understanding cellular metabolism and identifying potential metabolic bottlenecks or inefficiencies.

Protein-Nucleic Acid Interaction Studies: The labeled Xanthosine-[13C5] is used to investigate protein-nucleic acid interactions through techniques such as NMR spectroscopy. By observing how proteins interact with nucleosides, scientists can gain insights into structural and functional aspects of these interactions. This information is crucial for elucidating mechanisms of enzyme function and regulation.

Stable Isotope Labeling Studies: Xanthosine-[13C5] serves as a stable isotope tracer in various biochemical experiments. Its use allows researchers to study nucleotide turnover rates, synthesis pathways, and degradation processes. By examining these metabolic activities, the compound provides valuable data for developing therapeutic interventions related to nucleotide imbalances.

Radiolabel Replacement: Xanthosine-[13C5] offers a safer alternative to radiolabeled nucleosides in molecular biology experiments. By avoiding the use of radioactive materials, researchers can conduct labeling without handling hazardous substances. This approach enhances laboratory safety while providing reliable and reproducible results in studies involving nucleoside dynamics.