Stable isotope-labeled compounds are used as environmental pollutant standards for the detection of air, water, soil, sediment and food.
In addition to treating various diseases, isotopes are used for imaging, diagnosis, and newborn screening.
Small molecule compounds labeled with stable isotopes can be used as chemical reference for chemical identification, qualitative, quantitative, detection, etc. Various types of NMR solvents can be used to study the structure, reaction mechanism and reaction kinetics of compounds.
Stable isotope labeling allows researchers to study metabolic pathways in vivo in a safe manner.
The analysis team of BOC Sciences provides customers with high-quality impurity analysis services. Our experts are highly recognized by customers for their rich experience and proficient problem-solving skills.
Impurities refer to impure components contained in a substance. Their forms are solid granular, fibrous, soft jelly, liquid, and gaseous. Impurities are closely related to the production process and production process control level. Isotopic impurities have an important effect on the properties of substances. Therefore, it is very important to detect relevant impurities and control the purity for the development and production of isotope-labeled reagents.
Fig. 1 Impurities in the Spectrum
The isotope and impurity components in the sample are determined by gas chromatography, which is easy to operate and can meet many high-standard analysis requirements. The isotope sample analysis process can indirectly characterize the chemical purity of the isotope by means of the method of controlling the gas to be measured.
Use HPLC to separate the mixed samples of unknown impurities, obtain the isotopic peak shapes of quasi-molecular ions and fragment ions, and determine the elemental composition. Afterwards, it can be combined with other analytical instruments and equipment for structural identification and content analysis.
Inorganic MS can be used to measure the content of various elements and impurities in the sample. Isotopic MS can be used to measure the abundance ratio between different isotopes in a sample. Inorganic MS mainly includes inductively coupled plasma mass spectrometry (ICP-MS), time-of-flight mass spectrometry (TOF-MS) and so on. Isotopic MS mainly includes thermal ionization mass spectrometry (TIMS) and accelerator mass spectrometry (AMS).
HRMS data is a powerful tool in the analysis of unknown impurities, providing information on molecular weight, elemental composition and structure. In addition to calculating elemental composition, HRMS can also be used to unlock isotope information, calculate isotope ratios of unknowns, and obtain accurate impurity identification results.
Due to the different properties of compounds such as polarity and boiling point, some isotope-labeled compounds cannot be accurately calculated by conventional mass spectrometry. NMR spectroscopy can accurately reflect the chemical environment and structural site information of isotopic atoms. QNMR can use the internal standard method to measure the content of isotopic impurities in the sample through the integrated area of the spectral peak, and then obtain the isotopic abundance, obtain the information of the marked site, and calculate the impurity content.
BOC Sciences identifies and analyzes impurities through comprehensive separation and analysis methods, and provides a scientific basis for the development and production of isotope reagents with its mature technology in impurity research.