D-Cellobiose-[1-13C]

D-Cellobiose-[1-13C], a labeled disaccharide, finds diverse applications in research and industry. Here are the key applications:

Metabolic Studies: In metabolic flux analysis, D-Cellobiose-[1-13C] serves as a vital tool in unraveling carbohydrate metabolism pathways. By integrating this isotopically labeled cellobiose into experimental setups, researchers can meticulously monitor its conversion and distribution across various metabolic processes. This approach offers valuable insights into the dynamic nature of carbohydrate metabolism within living organisms, revealing the complex interplay of reactions and transformations.

Enzyme Mechanism Investigation: In enzyme studies, D-Cellobiose-[1-13C] plays a pivotal role in elucidating the mechanisms of cellulase and other carbohydrate-hydrolyzing enzymes. By utilizing this labeled substrate, researchers can detect and quantify enzymatic degradation products through advanced techniques like NMR spectroscopy and mass spectrometry. This methodology facilitates a detailed examination of enzyme kinetics, allowing the identification of key intermediate states in enzymatic processes, unveiling the molecular interactions within these cascades.

Structural Biology: In structural biology, D-Cellobiose-[1-13C] is valuable, particularly in Nuclear Magnetic Resonance (NMR) spectroscopy. Leveraging 13C labeling, scientists can conduct in-depth analyses of the molecular architecture and dynamics of cellobiose and its interactions with proteins and other biomolecules. This approach allows for a comprehensive exploration of binding sites and the functional mechanisms employed by carbohydrate-active enzymes, offering insights into the molecular dynamics that govern these essential biological interactions.

Gut Microbiome Research: In gut microbiome research, D-Cellobiose-[1-13C] is crucial for studying carbohydrate metabolism within the gut microbiota ecosystem. Researchers use this labeled compound to trace fermentation products and metabolic pathways utilized by microbial communities in the gastrointestinal tract. This research provides key insights into the roles of gut microbiota in shaping human health, revealing their impact on nutritional dynamics and metabolic homeostasis within the human body.