1,6-anhydro-β-D-galactose-[UL-13C6]

1,6-anhydro-β-D-galactose-[UL-13C6] is an isotopically labeled carbohydrate of interest in diverse biochemical and metabolic investigations. Here are the key applications:

Metabolic Flux Analysis: Delving into metabolic flux analysis, researchers employ 1,6-anhydro-β-D-galactose-[UL-13C6] to map carbon flow within metabolic pathways. Integrating this labeled compound into cell cultures, scientists track the destiny and recycling of galactose, shedding light on cellular metabolism and paving the way for targeted metabolic interventions. This data-rich approach unveils the intricate dance of molecules within the cellular landscape.

Glycan Biosynthesis Studies: Unraveling the mysteries of glycan biosynthesis, scientists utilize this labeled sugar to probe into glycan assembly within cells. By introducing 1,6-anhydro-β-D-galactose-[UL-13C6], researchers trace the incorporation of galactose into glycan structures via mass spectrometry, unraveling glycosylation pathways and the significance of specific glycans in biological and pathological contexts. This advanced technique offers a window into the elegant choreography of sugar molecules in cellular processes.

Isotope Dilution Assays: Serving as the linchpin in isotope dilution assays, 1,6-anhydro-β-D-galactose-[UL-13C6] acts as the internal standard for quantifying galactose levels in biological samples. By juxtaposing the signals of unlabeled and labeled galactose, researchers achieve precise quantification, a cornerstone in metabolic investigations in clinical and research settings. This methodological prowess empowers researchers to unravel the hidden complexities of metabolic pathways with accuracy and finesse.

Structural Biology: In the realm of structural biology, this compound takes center stage in unraveling carbohydrate-protein interactions. Leveraging 1,6-anhydro-β-D-galactose-[UL-13C6], researchers delve into NMR spectroscopy and other analytical tools to unravel the intricate dance of carbohydrates binding to proteins. This fundamental insight not only aids in the design of carbohydrate-based therapeutics but also enhances our understanding of how cells recognize and interact with their environment at a molecular level.