D-Glucose-[1-13C,1,2-18O2]

D-Glucose-[1-13C,1,2-18O2], an exquisitely labeled glucose molecule, stands at the forefront of cutting-edge research endeavors. Here are the key applications of D-Glucose-[1-13C,1,2-18O2]:

Metabolic Tracing: As a pivotal component in metabolic pathway investigations, this isotopically labeled glucose enables researchers to meticulously trace the complex flow of carbon atoms across a myriad of biochemical pathways. By integrating D-Glucose-[1-13C,1,2-18O2] into cells or organisms, scientists embark on a deep dive into the intricate process of glucose metabolism and the distribution of intermediates within metabolic networks. This profound knowledge is indispensable for unraveling the intricacies of metabolic disorders and devising precise metabolic interventions.

NMR Spectroscopy: Delving into the realm of nuclear magnetic resonance (NMR) spectroscopy, D-Glucose-[1-13C,1,2-18O2] emerges as a potent tool for exploring the structural dynamics of glucose and its derivatives within diverse biological systems. The presence of distinct 13C and 18O labels generates unique NMR signals, unveiling the atomic-level intricacies of glucose interactions and transformations. This technique aids in unraveling enzyme mechanisms and evaluating metabolic flux, offering invaluable insights into cellular processes at a molecular level.

Mass Spectrometry: Within the domain of mass spectrometry-based metabolic investigations, D-Glucose-[1-13C,1,2-18O2] shines as an indispensable tracer for quantifying metabolic fluxes and dissecting complex metabolic networks. The distinctive isotopic signatures of labeled glucose molecules enable precise measurement of metabolic rates and identification of specific metabolic intermediates. This application proves critical for advanced metabolomics studies and holistic systems biology research, facilitating a deeper understanding of metabolic processes and their implications.

Cellular Respiration Analysis: Transitioning towards the study of cellular respiration pathways, the utilization of labeled glucose offers a unique opportunity to trace the incorporation of 13C and 18O isotopes into metabolic byproducts such as CO2 and ATP. By observing the rate and pattern of isotopic integrations, researchers unveil profound insights into the efficiency and regulation of cellular respiration under varying physiological and pathological conditions. This analysis illuminates the intricacies of energy metabolism and aids in identifying potential therapeutic targets for metabolic disorders, fostering advancements in precision medicine.