D-fructose-[1-13C] 1,6-bisphosphate sodium salt

D-fructose-[1-13C] 1,6-bisphosphate sodium salt, a stable isotope-labeled compound widely utilized in biochemical research, holds key applications across various domains. Here are the significant applications:

Metabolic Flux Analysis: Delving into metabolic pathways and fluxes through glycolysis and gluconeogenesis, scientists leverage D-fructose-[1-13C] 1,6-bisphosphate sodium salt as a pivotal tool. By tracing the 13C label through intricate metabolic networks, researchers unravel the flow of carbon atoms during cellular processes, shedding light on enzyme activities and regulatory mechanisms within these pathways.

NMR Spectroscopy: A staple in nuclear magnetic resonance (NMR) spectroscopy, this compound plays a crucial role in probing the structure and dynamics of biomolecules. The presence of the 13C label yields a distinctive signal in NMR spectra, facilitating in-depth analyses of carbohydrate metabolism and molecular interactions. Through this technique, researchers glean insights into molecular conformation and the interplay between enzymes and substrates.

Metabolic Disorders Research: In the realm of metabolic disorders, D-fructose-[1-13C] 1,6-bisphosphate sodium salt emerges as a valuable asset for comprehending the biochemical perturbations associated with conditions like diabetes and fructose intolerance. By monitoring the processing of the labeled compound in diseased versus healthy cells, scientists pinpoint potential metabolic dysfunctions and therapeutic targets. This contributes significantly to the tailored development of diagnostic and treatment strategies.

Tracer Studies: Employed in tracer studies to monitor carbohydrate metabolism in both in vitro and in vivo settings, D-fructose-[1-13C] 1,6-bisphosphate sodium salt is a cornerstone for understanding metabolic activities. By integrating the labeled compound into experimental setups, researchers track its conversion and utilization in metabolic pathways. This application is indispensable for investigating the impacts of genetic alterations and pharmacological interventions on metabolic function, paving the way for enhanced understanding of metabolic dynamics.