α-D-galactopyranosyl 1-phosphate-[1,2,3-13C3] (dipotassium salt) is a labeled sugar phosphate used in various bioscience research applications. Here are some key applications of α-D-galactopyranosyl 1-phosphate-[1,2,3-13C3]:
Metabolic Flux Analysis: This labeled sugar phosphate is an essential tool for tracing metabolic pathways in cells. By using the 13C isotope, researchers can monitor the incorporation and distribution of the compound in metabolic networks via NMR or mass spectrometry. This allows for a detailed understanding of carbohydrate metabolism and its alterations in different conditions.
Isotope Tracer Studies: α-D-galactopyranosyl 1-phosphate-[1,2,3-13C3] is used in isotope tracer experiments to determine the rate of metabolic processes. The 13C labeling enables precise measurement of the dynamic flow of carbon atoms through metabolic pathways. This information is critical for elucidating biosynthetic routes and determining the fate of metabolites.
Glycosylation Pathway Research: In glycosylation studies, this labeled compound helps to investigate the biosynthesis and processing of glycoconjugates. By tracing the labeled sugar phosphate through cellular processes, scientists can examine how glycosyltransferases and other enzymes alter these molecules. This insight is vital for understanding disorders related to defective glycosylation.
Structural Biology: α-D-galactopyranosyl 1-phosphate-[1,2,3-13C3] is valuable in structural studies of carbohydrate-active enzymes. The 13C labels facilitate the use of nuclear magnetic resonance (NMR) spectroscopy to determine the three-dimensional structures and dynamics of enzyme-substrate complexes. This helps in deciphering the molecular mechanisms of enzyme action and improving enzyme engineering.
![α-D-galactopyranosyl 1-phosphate-[1,2,3-13C3] (dipotassium salt)](https://resource.bocsci.com/structure/d-1-2-3-13c3-galactopyranosyl-1-phosphate-dipotassium-salt.gif)
