D-mannose-[1,2,4-13C3]

D-mannose-[1,2,4-13C3], an isotopically labeled sugar, finds intriguing applications across diverse scientific research domains. Here are its key applications:

Metabolic Flux Analysis: In the quest to unravel cellular intricacies, D-mannose-[1,2,4-13C3] proves invaluable for dissecting metabolic pathways and exploring carbohydrate metabolism. By tracing the movement of labeled carbon atoms, researchers can scrutinize sugar utilization through various enzymatic routes. This analysis provides profound insights into cellular energy generation and reveals metabolic anomalies, offering a clearer picture of how cells manage and process carbohydrates.

Cellular Transport Studies: At the cutting edge of scientific inquiry, D-mannose-[1,2,4-13C3] is employed to investigate sugar transport mechanisms across cellular membranes. By tracking the uptake and distribution of labeled mannose, researchers can elucidate the roles of specific transport proteins and the fundamental pathways involved in carbohydrate trafficking. This investigation not only enhances our understanding of cellular transport but also identifies potential therapeutic targets for conditions related to carbohydrate metabolism disruptions.

Glycosylation Research: In the complex realm of biochemistry, D-mannose-[1,2,4-13C3] emerges as a key player in studying glycosylation—where sugars conjugate with proteins or lipids. Incorporating labeled mannose into glycoproteins allows researchers to decipher the intricacies of glycoprotein biosynthesis and modification. This application is crucial for understanding disorders like congenital glycosylation abnormalities and provides insights into the detailed processes of cellular glycosylation.

Stable Isotope Labeling in NMR Studies: Leading the way in structural investigations, D-mannose-[1,2,4-13C3] finds significant utility in Nuclear Magnetic Resonance (NMR) spectroscopy. The unique signals from the labeled carbons facilitate detailed analysis of carbohydrate interactions and molecular conformations. This application enriches structural biology research, aiding in the development of carbohydrate-based therapeutics and enhancing our understanding of molecular architectures and functions.